Drosophila s2actwgpuro cell line was established by transfected pacwg and pbspuro in ratio of 1. This guide is adapted from the university of arizona department of biochemistry and molecular biophysics general biology program for science teachers. Our ap biology lab activities are designed to provide a wide variety of experiences. Fly genetics are a huge part of the field, the tiny little drosophila melanogaster providing aspiring geneticists with over a century of good times. Research of these flies initially entered labs 100 years ago. For my set of flies we concluded that there was a recessive autosomal trait for vestigual wings that was not present in the f1 generation but in the f2 generation. On this page you can read or download ap biology lab 7 genetics of drosophila virtual version answer key in pdf format. The purpos of this lab was to view autosomal and sexlinked traits and how they were passed through generations lab conclusion. Of these factors, temperature is the most important. Drosophila melanogaster introduction drosophila mature through complete metamorphosis, as do all members of the order diptera. Ap biology lab 7 genetics of drosophila virtual version. Genetics of dr o s o p h i l a virtual version overview.
This weighty book was written with the objective of providing a summary of the practice of drosophila genetics genetics subject category. Ap biology lab 7 genetics of drosophilia answer key. One good source of information on drosophila is the drosophila manual. And flies are also a royal pain in the butt to work with. Drosophila melanogaster, the fruit fly, is a great organism for genetic use because it has simple food. Showing top 8 worksheets in the category ap biology lab 7 genetics of drosophilia answer key.
Genetics of organisms ap biology, lab 7 multimedia cd 2005. The book s appendices include key aspects of drosophila biology, essential solutions, buffers, and recipes. However, one of the mutations is sex linked sepia eyes, while the other is autosomal vestigial wings. Some of the worksheets displayed are ap biology lab 7 genetics of drosophila virtual version, ap biology lab 7 genetics of drosophila virtual version, big genetics and information transfer 3, lab 9 principles of genetic inheritance, ap biology lab 12 tm, biology practice exam, exercise 7, ap biology. This allows for simple monohybrid crosses, sexlinkage, gene linkage and many other combinations so. The original format is maintained, providing a handy reference guide to most aspects of the biology of drosophila. Introduction to drosophila melanogaster model system. Here is a short guide to the biology and culture of drosophila melanogaster. Review and cite drosophila biology protocol, troubleshooting and other methodology information contact experts in drosophila biology to get answers. The history of fruit flies is considered a tradition. You will learn how to collect data from f1 and f2 generations and analyze the results from a monohybrid, dihybrid, or sexlinked cross. The investigation pulls together topics such as genetics, animal behavior, development, plant and animal structures from cells to organs, cell communication, fermentation, and evolution. After showing themselves to be qualified on the ap exam, some students, as college freshmen, are permitted to take upperlevel biology courses or register for courses for which biology is.
Thomas preformed his research in morgan lab at the columbia university in 1910. They should not be confused with the tephritidae, a related family. Drosophila melanogaster and mendelian genetics, by pete geiger. Genetics of drosophila virtual version overview in this lab you will be doing virtual genetic crosses of fruit flies drosophila melanogaster. Students will make hypotheses for monohybrid, dihybrid and sexlinked traits and test their hypotheses by selecting fruit flies with different visible mutations, mating them, and analyzing the. Biology of drosophila was first published by john wiley and sons in 1950. This lab requires two to four 50minute class periods. The advanced placement biology course is the equivalent of a twosemester college introductory biology course taken by biology majors during their first year. Drosophila melanogaster, the fruit fly, is an excellent organism for genetics studies because it has simple food requirements, occupies little space, is hardy, completes its life cycle in about 12 days at room temperature, produces large numbers of offspring, can be immobilized readily for. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Includes access to exclusive flinnprep digital content to combine the benefits of classroom, laboratory and digital learning.
Apterous ap is recessive and is located on chromosome 2. Thomas hunt morgan began using fruit flies in genetic studies at columbia university in 1910. The life cycle of drosophila melanogaster it is important to realize that a number of factors determine the length of time of each stage in the lifecycle. An evolution of michael ashburners 1989 classic drosophila.
One good source of information on drosophila is the drosophila manual, available from carolina biological. Models is the fruit fly drosophila melanogaster owing to the abundance of protocols. Drosophila melanogaster lab report essay 1290 words. The second edition of the highly successful grey book drosophila. Some of the worksheets displayed are ap biology lab 7 genetics of drosophila virtual version, ap biology lab 7 genetics of drosophila virtual version, big genetics and information transfer 3, lab 9 principles of genetic inheritance, ap biology lab. Early experiments conducted in the lab of thomas morgan uncovered many interesting drosophila phenotypes.
However, original copies are now very hard to find. This is an excellent adaptation for the college board lab on genetics. The lab includes a thorough background, detailed procedure including tips on immobilizing, sexing and phenotyping flies. Disciplines, occupations and industries see more details and biology biology subject category. During this lab, we were to use fruit flies to do genetic crosses, learn how to determine the sex of fruit flies and recognize contrasting phenotypes, and collect data from f 1 and f 2 generations and analyze the results of. Drosophila melanogaster is used in this lab as well as many other wetlab experiments, particularly genetic experiments, because it meets all the criteria in order to be a model organism. For about 1 month, the cells grow healthily and the cell line was established. Formula 424, sorting brushes, sorting cards, and a carolina drosophila manual. Stay connected to your students with prezi video, now in microsoft teams. We have kits for new and traditional ap biology labs.
Drosophilab this program must be downloaded and then run from a computer or server, not quite as buggy as the vcis program is. Understand the genetic approach to biological problems and the use of molecular biology. Drosophila genetics applying mendelian principles through. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. In model organisms such as the common fruit fly, drosophila melanogaster. For use in ap biology, college biology or advanced biology. Genetics of drosophila overview in this lab you will be doing virtual genetic crosses of fruit flies drosophila melanogaster. Each blended learning lab solution includes prelab videos about concepts, techniques. Software at allows for multiple matings and statistical analysis. Ap biology lab 7 genetics of drosophila lab activity 36 w 7105 36 w 7116. From the carolina drosophila manual, carolina biological. Disciplines, occupations and industries see more details. Part of the reason people work on it is because it is a small animal, with a short life cycle of just two weeks, nd is cheap and easy to keep large numbers. Students will learn and apply the principles of mendelian inheritance by experimentation with the fruit fly drosophila melanogaster.
It comes with every order of fly material and is often given away free at conferences. Drosophilab is a brilliant, free and downloadable piece of software that allows students and teachers to edit fruit flies and carry out crosses. Until its appearance, no central, synthesized source of biological data on drosophila melanogaster was available, despite the flys importance to science for three decades. The manual is an excellent source of information on many topics. Ap bio lab 7 genetics of drosophila bozemanscience. Their relatively fast life cycle and their ease of use made this common fruit fly an important historical model species, and they are still studied by geneticists today. Ap biology fruit fly lab the genetics of drosophila. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. A laboratory handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. Fruit fly genetics very simple program with limited capabilities, useful for showing f1 and f2. Drosophila flies carry three sets of autosomes and two sex chromosomes.
Download ap biology lab 7 genetics of drosophila virtual version answer key document. The teacher can use the chromosome editor to set up parent flies of any genotype and there are 20 genes and traits represented, on four chromosomes. Im having trouble creating a hypothesis on the outcome of the f2 generation. Prepare your students for medical and lab tech careers with carolina s wide range of. Drosophila melanogaster, the fruit fly, is an excellent organism for genetics. The emphasis is on experimental methods, the authors aim being to provide a methodological. Thomas hunt morgan, who lived from 1866 to 1945, was the founder of drosophila genetics. Were doing a lab in my ap biology class that requires mating fruit flies with two different mutations dihybrid cross and observing the phenotypes of the f2 generation. This was the final project for our ap biology class. You might call them and ask if they would be willing to send you one. Practical uses in cell and molecular biology is a compendium of mostly short technical chapters designed to provide stateofthe art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. Ten years in the making, it was an immediate success and remained in print for two decades. Drosophila has been used as a model organism for research for almost a century, and today. By breeding fruit flies drosophila melanogaster of unknown genetic composition and studying the traits and ratios seen in their offspring, you will determine.
Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. A laboratory manual, this book is an essential addition to the personal library of drosophila investigators and an incomparable resource for other research groups with goals. Drosophila melanogaster is a small, common fly found near unripe and rotted fruit. See more ideas about genetics, fruit flies and microscopic photography. If you dont see any interesting for you, use our search form on bottom v. Drosophila genetics labs without the the need to select virgin female flies for crosses. Ap biology pill bugdrosophila behavior lab youtube. His laboratory was located on the top floor of schermerhorn hall, which became known as the fly room.